Extramammary Paget's disease of the scrotum: report of 79 cases / 中华肿瘤杂志

<p><b>OBJECTIVE</b>To investigate the clinicopathologic feature and treatment result as well as prognosis of Paget's disease of the scrotum.</p><p><b>METHODS</b>The data of 79 patients with Paget's disease of the scrotum confirmed pathologically from 1993 to 2004 were retrospectively analyzed. The median age of these patients was 62.3 years. The median duration of the disease was 3 years and 7 months. Locally extensive excision and intra-operative frozen section analysis of resection margin were performed in 78 patients. Inguinal and iliac radical lymphadenectomy was done in 7 patients with inguinal lymph node metastasis.</p><p><b>RESULTS</b>Local recurrence developed in 9 patients, and 3 patients died of the disease during follow-up.</p><p><b>CONCLUSION</b>The prognosis of Paget's disease of the scrotum after surgical resection is good. To highly watch out for the disease, to perform histological examination and surgical resection as early as possible are keys to control it successfully.</p>

Characteristics of proliferation and myotube cell formation of rat skeletal muscle satellite cells cultured in vitro / 上海第二医科大学学报

Objective To establish a method of isolation and purification of rat skeletal muscle satellite cells,and observe the characteristics of proliferation and myotube cell formation of skeletal muscle satellite cells cultured in vitro. MethodsPurified skeletal muscle satellite cells were obtained by improved two-step enzymatic digestion method and pre-plating technique.Immunohistochemical staining was employed to identify the skeletal muscle satellite cells cultured in vitro.The growth of skeletal muscle satellite cells cultured in vitro was examined by MTT assay.The differentiation of skeletal muscle satellite cells was observed by inverted microscopy. Results The skeletal muscle satellite cells with higher purity were obtained and confirmed by the high expression of desmin.When cultured in vitro,the latent phase of skeletal muscle satellite cells was the first to the second day,and the platform phase was the fifth to the sixth day.Myotube cells gradually formed when cell confluence was more than 60% to 70% or differential medium with lower fetal bovine serum was used.Conclusion The combination of improved two-step enzymatic digestion method and pre-plating technique serve as an easy and practical way to obtain skeletal muscle satellite cells with higher purity.Skeletal muscle satellite cells can form myotube cells with contraction characteristics without any special induction.

Relationship between stress urinary incontinence and apoptosis of urethral peripheral muscle cells / 上海第二医科大学学报

0.05). Conclusion The birth injury has little impact on the apoptosis of urethral peripheral muscle cells,while the estrogen decrease may increase the cell apoptosis.